universal primer meaning in Chinese
通用引物
万能底漆
Examples
- Also , these str loci were amplified by multiplex pcr using our primer set , but the universal primers were fluorescent - labeled . the products of the multiplex pcr were genotyped by abi prism 310 genetic analyzer
结果通过采用公共引物和基因座特异性嵌合引物的方法,建立了法医str基因座嵌合引物复合扩增方法。 - Result . a method of multiplex pcr based on both chimeric and universal primers was established . a fluorescent quadriplex str system , including d1s1612 , d9s2026 , d20s161 and d6s477 , was developed on the basis of the multiplex pcr
建立了dis1612 、 d952026 、 d205161 、 d65477str基因座荧光标一记嵌合引物复合扩增毛细管电泳激光自动分析体系,同时证明嵌合引物复合扩增方法与毛细管电泳激光自动分析方法相兼容。 - Based upon the comparison of cyto b gene sequences in 15 deer species downloaded from genbank , a universal primer set l15774 / hsf21 was used as positive control of the template quality , at the meantime , two species specific primer sets df / dr and cf / cr were desgined to identify red deer ( cervus elaphus ) , sika deer ( cervus nippori ) and roe deer ( capreolus capreolus ) from other species . a musk deer ( moschus ) specific primer set wf / mr was designed , siberian musk deer ( afoschus moschiferns ) and forest musk desr ( moschus berezovskii ) could be discriminated when restriction endonuclease rsa i was used to cut the pcr products
经过对来自genbank中的15种鹿类动物的cytob基因序列的比较,用通用引物l15774和hsf21作为模板的质量控制,设计了特异性引物df dr和cf cr来鉴定马鹿、梅花鹿、狍;设计了麝类特异性引物mf mr ,用限制性内切酶rsa酶切扩增产物来区分原麝和林麝。 - In this paper , total dna from tannage , tail skin , scales and the skin treated with salt were successfully extracted with an improved method for dna extraction . to verify the results , four pairs of primers , which were universal primers for 12s rrna gene , diagnostic primers of chinese alligator meat , microsatellite primers and rapd primer , were used to do pcr amplifications . some amplified fragments were sequenced , either
本文研究出一种改进的dna提取方法,成功地从扬子鳄鞣制皮革中提取了总dna ,同时对不同的组织标本如鳞片、盐腌生皮及尾尖皮等进行了dna提取:并利用12srrna基因扩增的通用引物、扬子鳄鉴定性引物、微卫星引物及rapd引物进行pcr扩增,且对部分pcr扩增结果进行测序,以检验提取效果。